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SLQ-6A Coarse Fiber Determinator Usage Manual

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Ⅰ. Summary

SLQ – 6A coarse fiber determinator is a set of the instrument

for determining coarse fiber to adopt chemistry and physics technology .The instrument

is applicable to analyze coarse fiber content in food, animal feed and other

agriculture by-products.

The design principle of the instrument is based on

acid and alkali washing method. It gathers the function to handle and flushed

with sour or alkali, is adopted a closing construction and electricity heat method,

which can determine 6 samples at the same time, does not need to transfer

sample, can resolve out fiber in the material. It as well as can regulate

arbitrarily the temperature needed for the single sample. That attains an

accurate and fast purpose. During operate, may control timing, doesn’t overflow

the harmful material, so insure that the test data is dependable and veracious

and be beneficial to the environmental decontamination, operate is convenience for

personnel. Consequently the instrument is an ideal instrument to determine coarse

fiber for the institute, Industry of food and animal feed, also is the instrument

of experience and education for university and college.

Ⅱ. Working Principle

Using definite concentration acid or alkali digests

sample under the particular condition, then using ether and alcohol removes the

material dissolving ether, ignite at high temperature and deduct mineral,

residual material is called as coarse fiber. It is not an accurate chemistry composition,

just in accepted rule measure a compendia composition which contains mainly fiber,

a little half cellulose and lignin.

Ⅲ. Technique Parameter

1. Measurement scope: Coarse fiber content in all kind

of food, animal feed and other agriculture by-products.

2. Measurement accuracy: ≤0.2%.

3. Re-appear: The parallelism test result ≤0.4%.

4. Measurement time: 90min/ batch.

5. Test sample number at the same time: 6/ times

6. Power supply: 220 (V)±10﹪; 50HZ.

7. Power: 2KW

8. Shape size: 560×520×640 mm3.

9. Weight: 30kg

Ⅳ. Usage Elucidation

1.  Apparatus

Coarse fiber determinator;

Analyze balance: min 0.0001 g;

Electrical oven (1500W, 50-150℃);

Desiccator with the silicon of changing color, tow

sets;

muffle furnace (2KVA, 200-1000℃);

Pulverizer or mortar for the laboratory;

Screen: 0.45

mm2 aperture (40 mesh)

2. Reagents

Sulfuric acid (H2SO4), AR;

Sodium hydroxide (NaOH),AR;

95% Alcohol, AR;

Ether, AR;

N-octanol, AR;

Diatomaceous earth (filter aid)

Acetone

Litmus paper.

3. The operation preparation  

a. Reagents preparation

1, a solution of sulfuric acid (0.128mol / L): Take

concentrated sulfuric acid (analytical grade) to about 69 mL, diluted to 1000ml

with distilled water, dried calibration solution of known concentration of

NaOH, their concentration adjusted to 1.28 ± 0.005mol / L of liquid sulfuric

acid, can be diluted 10-fold with distilled water before use.

2, an alkaline solution (0.312mol / L): Take about

174ml clarify NaOH solution saturated with CO2-free distilled water to dilute

1000ml Gasser oscillation even with a known concentration of acid after

calibration standard solution to adjust its concentration to 3.12 ± 0.005 mol /

L of a base liquid, can be diluted 10-fold with distilled water before use.

B, sample preparation

1, take a representative sample, pick impurities,

according to quartering take about 25g.

2, the sample was dried in a home 60 ~ 65 ℃ oven for about 8 hours, cooled and

pulverized or grinder, all through 0.84mm2 (20 mesh) sieve, wherein greater

than 0.45mm2 (40 mesh) is not less than 50%; less than 0.25mm2 (60 mesh) shall

not exceed 10%.

3, the fat content of the sample more than 1%

defatted (available residue was determined after analysis of the fat, no fat).

4, the crucible was washed with water, to wash the

crucible without leaving any impurities, placed in a drying oven for about 1

hour (at a temperature of 105 ℃) moved into a desiccator to cool to room temperature, serial

number, and then placed in the oven within the reserve.

(D), the steps

1, in the crucible numbered after the first 1g of Celite, and then accurately

weighed sample average net dry or drying anhydrous defatted sample 1-3g

(cereals 2-3g) accurate to 0.0001g.

2, power, water supply, turn the mains switch (open water as far as possible to

ensure full condensation effect).

3, respectively, the corresponding acids, bases, water hose is connected

properly and host cooking liquor is poured into a solution with a good right

amount (about 1L), turn on the power.

4, open acid, alkali, water preheating power small switch, pre-heated to a boil

(about 80 ℃).

5, the crucible containing the sample transferred to a greenhouse instruments

(note this time remains accurate unbiased position: the lower part of the

crucible into the center seat, the upper part of the hole is aligned Digestion

tube under protective cover; Press the handle and lock check again after tight

crucible position is accurate, the lower valve all closed, pulled out one by

one heater handle.

6. Acid Digestion: first open 1-6 Dosing valve, open acid

valve, then open the dosing switch, has been preheated to about 5ml H2SO4

solution were added to the crucible, after the addition of switching off dosing

switch, and then open the suction switch, one by one under the open valve,

exhausted solution, sucked off all the switches and lower valve. Then H2SO4 has

been preheated to the mark in the middle line (about 150ml), and then add 8 to

10 drops in each Digestion tube octanol, start the timer, respectively, at the

same time as needed to open heater power switch, the selected bit adjustment

knob  can be observed a single heater

voltage. And raised to the highest voltage (220V). Digestion liquid boil until

the voltage down individually adjusted to the heating temperature of the sample

without precipitation, start timing knob to 30min, keeping Digestion simmering

liquid (such as found in the sample sticking to the wall can eliminate

additional cooking liquor). When heating is automatically stopped.

7, open the suction switch, and then one by one under the open valve  Digestion was deprived of rapid (within 10min

exhausted) and washed with hot distilled water to neutral residue (about three

times), the exhausted wash liquid, such as found in the leaching process is

blocked off suction when the crucible switch, blow open the switch  with a jet recoil found after bubbles appear

within Digestion tube blow off switch, open the switch continues to suck sucked

sucking liquid after closing the suction switch.

8 Press (6) Step alkali Digestion.

9, press (7) Step extractor alkali cooking liquor consumption.

10, bleaching and degreasing, all closed lower valve,

with a fat belly straw respectively Digestion tube catchy added in three

portions of 95% ethanol, soak, filtration (each about 20ml, each immersion time

of about 1min), then added in three portions of ether, rinse (each about 20ml),

(Ruoyi defatted sample does not require rinsing with diethyl ether).

11, rinsed with acetone sample 3 times, washed once with distilled water. (Each

20ml)

12, the left hand pressing the handle locking hook pulled slowly rising, it is

reset with gloves, remove the sample crucible there until the ether and ethanol

all volatiles finished, then shifts in oven at a temperature of 130 ℃, bake 2 hours, was brought out in a desiccator to cool

to room temperature, the weighed (A1).

13, the crucible after weighing, put into a furnace burning 500 ℃ resistor 30min, (must be placed in a cold crucible

furnace, followed by heating) until the furnace temperature dropped to below

200 deg.] C, was brought out in the resistance furnace after weighing

desiccator cooling to room temperature (A2).

14, the measurement results calculated

CF% = (A1-A2) ÷ S1 × 100

Where: CF- percentages of crude fiber (%)

      A1- crucible + + crude fiber and ash

residue mass (g)

      A2- crucible + ash residue and mass (g)

      S1- sample weight (g)

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